Appendix for Glyoxal as an alternative fixative to formaldehyde in immunostaining and super-resolution microscopy
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چکیده
Glyoxal as an alternative fixative to formaldehyde in immunostaining and super-resolution microscopy Katharina N. Richter, Natalia H. Revelo, Katharina J. Seitz, Martin S. Helm, Deblina Sarkar, Rebecca S. Saleeb, Elisa D’Este, Jessica Eberle, Eva Wagner, Christian Vogl, Diana F. Lazaro, Frank Richter, Javier Coy-Vergara, Giovanna Coceano, Edward S Boyden, Rory R. Duncan, Stefan W. Hell, Marcel A Lauterbach, Stephan E. Lehnart, Tobias Moser, Tiago Outeiro, Peter Rehling, Blanche Schwappach, Ilaria Testa, Silvio O. Rizzoli
منابع مشابه
Glyoxal as an alternative fixative to formaldehyde in immunostaining and super‐resolution microscopy
Paraformaldehyde (PFA) is the most commonly used fixative for immunostaining of cells, but has been associated with various problems, ranging from loss of antigenicity to changes in morphology during fixation. We show here that the small dialdehyde glyoxal can successfully replace PFA Despite being less toxic than PFA, and, as most aldehydes, likely usable as a fixative, glyoxal has not yet bee...
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Tissue fixation in phosphate buffered formalin (PBF) remains the standard procedure in histopathology, since it results in an optimal structural, antigenic and molecular preservation that justifies the pivotal role presently played by diagnoses on PBF-fixed tissues in precision medicine. However, toxicity of formaldehyde causes an environmental concern and may demand substitution of this reagen...
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Immunostaining and fluorescence in situ hybridization (FISH) are well established methods for specific labelling of chromatin in the cell nucleus. COMBO-FISH (combinatorial oligonucleotide fluorescence in situ hybridization) is a FISH method using computer designed oligonucleotide probes specifically co-localizing at given target sites. In combination with super resolution microscopy which achi...
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The condensation of glyoxal with benzylamine in the presence of formaldehyde leads to 2,4,6,8-Tetrabenzyl-2,4,6,8-Tetraazabicyclo [3.3.0] octan (6) in methanol. The scopes and limitations of the reaction have been determined. At low temperatures, a mixture of (6) and diol (7) is formed.
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